Química Farmacéutica · 2018

Estudio fitoquímico de la especie vegetal Bucquetia glutinosa (L.f.) DC (Melastomataceae) y evaluación de su actividad biológica

Thanks to the biodiversity presented by the plants and the multiple benefits attributed to them, they have become a source of important studies for worldwide development. In this research study we wanted to identify the metabolites present in the crude extract of the plant species Bucquetia glutinosa (Melastomataceae), as well as its toxicity in order to establish a potential use of this species. Bioassays were carried out with four fractions of the crude extract (ethanolic, butanolic, chloroformic and hexane) using four microorganisms (S. aureus, E. coli, Klebsiella sp and candida sp) to determine the biological activity that could occur B. glutinosa. According to the in vitro tests, it was identified that the endemic species of the city of Bogotá presents secondary metabolites such as alkaloids, triterpenoids and / or steroids, saponins, phenolics / tannins, flavonoids and quinones, which were identified by means of different tests. coloration and precipitation in a preliminary phytochemical analysis, an LC50 of 133,593 μg / mL was also determined, thus presenting a moderate degree of toxicity. The bioassays evaluated showed that the crude extract and its fractions of B. glutinosa show high activity against S. aureus bacteria, intermediate activity against yeast Candida sp and null activity against E. coli and Klebsiella sp. By determining the minimum inhibitory concentration, it was observed that the minimum concentration that inactivates the microorganism is 30mg / ml and the bioautography test concluded that phenolic compounds, flavonoids and tannins may be responsible for the antibacterial activity that it was evidenced in this research project.

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