Bogotá - Ciencias - Doctorado en Ciencias - Bioquímica · 2022

Aproximación transcriptómica y fisiológica para el estudio de los mecanismos moleculares involucrados en la interacción clavel (Dianthus caryophyllus L.) – Fusarium oxysporum f. sp. dianthi

Vascular wilting caused by Fusarium oxysporum f. sp. dianthi (Fod) in carnations is undoubtedly one of the diseases with the greatest impact on the world flower sector. The molecular study of this plant-pathogen interaction will make it possible to propose new strategies for its early diagnosis and control in crops, and provide tools for the genetic design of varieties resistant to the pathogen. This study presents the first joint study of physiological parameters and comparative transcriptomics using RNAseq in this pathosystem. From the physiological approach, it was found that the mechanisms displayed by the plants of the resistant variety under study included stomatal closure without affecting the production of total chlorophyll, photochemical efficiency of photosystem II, or biomass production. Likewise, it was found that, at least in the varieties studied, leaf temperature can be postulated as an indicator for early diagnosis of the disease. On the other hand, the molecular mechanisms associated with resistance against Fod and displayed by the roots at early times include the activation of genes that code for potential RGA resistance proteins, oxidative burst, cell wall biogenesis, biosynthesis of specialized metabolites, chloroplast metabolism, and, in general, the transcriptional regulation related to stress response genes, defense response, and, to a lesser extent, some genes that participate in the response to osmotic stress, as well as some genes of hormonal pathways. In general, the transcriptional response, which was partially validated by RT-qPCR, is complex and shows components that are reported for the first time in the carnation response to the causal pathogen of vascular wilting.

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