Química Farmacéutica · 2018

Estudio fitoquímico de la especie vegetal Solanum crinitipes Dunal (Solanaceae) y evaluación de uso como agente antimicrobiano

Solanum crinitipes is a species native to the Andes from Colombia to Peru and was collected in the Mochuelo Alto Village of Ciudad Bolívar (Bogotá D.C). The ethanolic extracts obtained from the fruits, leaves and stems were subjected to preliminary phytochemical analysis and were also evaluated against bacteria such as Escherichia coli and Staphylococcus aureus and fungi such as Aspergillius sp, Botrytis sp, Candida sp, Fusarium sp, Penicillium sp, Rhizopus sp, and Trichoderma sp. From the preliminary phytochemical analysis, the presence of secondary metabolites such as alkaloids, triterpenoids, steroids, phenolics, tannins, coumarins, lactonic compounds and quinones was established. The extracts showed moderate toxicity against Artemia salina. In the tests of antimicrobial activity, using the technique of diffusion by wells, it was evidenced that the leaf extract has a medium activity against S. aureus. The leaf extract, being the most active, was fractionated by Soxhlet extraction using chloroform, acetone and ethanol as solvents. The obtained fractions were evaluated again with S. aureus and the results showed that the fraction in acetone shows a high activity while the fraction in chloroform showed an average activity. The Minimum Inhibitory Concentration of the fraction in acetone was 30 mg / mL at which a decrease in the growth of S. aureus was observed. The acetone fraction contains among its metabolites alkaloids, tannins and cardiotonic compounds that may be associated with antimicrobial activity.

Tesauro de su biblioteca